Table 1. EvaGreen RT-qPCR analysis of select genes across placental datasets.

Gene	Gene Description	Probe	Description	Day	N	Std ErrMS	Std ErrWC	Fold Change(MS-WC)	Student t-Test ΔCt (Normalization)
ABCA1	ATP-binding cassette transporter member 1	Ssc.7146.A1_a1	Cholesterol Efflux	25	6	0.14	0.30	−6.63	3.11E–4§
				45	6	0.10	0.21	−3.65	2.55E–3§
				65	6	0.24	0.86	−4.40	7.15E–4§
				85	6	0.08	0.63	−5.32	6.21E–3§
				105	6	0.17	0.60	−8.15	3.955E–3§
XIST *	X (inactive)-specific transcript	Ssc.2434.1.A1_at	X-Cs inactivation	25	6	0.68	0.42	−1573.8	3.08E–02§
				45	6	0.19	0.25	−14.02	5.38E–08§
				65	6	0.60	0.96	−138.14	4.28E–02§
				85	6	0.31	0.69	−2149.8	3.82E–02§
				105	6	0.44	0.41	−576.03	1.47E–02§
XIST *	X (inactive)-specific transcript	Ssc.31029.1.A1_at	X-Cs inactivation	25	6	0.30	0.56	−2.19	5.11E–06§
				45	6	0.45	1.00	−1.84	1.46E–04§
				65	6	0.59	0.95	−1.12	3.56E–02§
				85	6	0.35	0.28	−4.72	1.82E–02§
				105	6	0.25	0.23	−1.68	3.67E–02§
PHLDA2	pleckstrin homology-like domain, family A, member 2	Ssc.9796.1.A1_at	Genomic Imprinting	65	6	0.57	0.82	2.27	1.48E–02§
CDKN1C	cyclin-dependent kinase inhibitor 1C (p57, Kip2)	Ssc.8871.1.S1_at	Genomic Imprinting	65	6	0.15	0.29	1.82	4.08E–06§
RPS20	ribosomal protein S20	Ssc.20036.1.S1_at	Internal Reference	65	6	0.49	0.07	–	–
RPL18 *	ribosomal protein L18	Ssc.10553.1.A1_a_at	Internal Reference	25	6	0.38	0.83	–	–
RPL18 *	ribosomal protein L18	Ssc.10553.1.A1_a_at	Internal Reference	45	6	0.43	0.50	–	–
RPL18 *	ribosomal protein L18	Ssc.10553.1.A1_a_at	Internal Reference	65	6	0.62	0.91	–	–
RPL18 *	ribosomal protein L18	Ssc.10553.1.A1_a_at	Internal Reference	85	6	0.21	0.44	–	–
RPL18 *	ribosomal protein L18	Ssc.10553.1.A1_a_at	Internal Reference	105	6	0.26	0.51	–	–

^*Denotes ΔC_t values normalized with RPL18.

^§Denotes significances p<0.05.