Table 3.
Key cytokine and signaling pathway elicited in response to CBHA at 6h and 24h in H9c2 cells*
|
Control versus CBHA (6 h) |
Control versus CBHA (24 h) |
||
|---|---|---|---|
| Gene pathway | Total connections (No. of focus genes) | Gene pathway | Total connections (No. of focus genes) |
| TNF |
45 (22) |
CDKN1A |
65 (35) |
| Myc |
43 (18) |
TP53 |
60 (35) |
| HNF4A |
32 (18) |
IL-6 |
50 (34) |
| PTEN-PI3K-Akt |
76 (17) |
HNF4A |
41 (30) |
| ERK-p38-JNK |
73 (17) |
CDKN2A |
43 (25) |
| NFκΒ |
40 (16) |
ERK- p38-JNK |
52 (21) |
| IFNγ |
45 (12) |
NFκΒ |
41 (17) |
| FOS |
43 ( 8 ) |
IFNγ |
38 (17) |
| AP1 |
31 ( 8 ) |
Cyclin A |
29 (12) |
| Insulin |
28 ( 8 ) |
PI3K-Akt |
41 (10) |
| TP73 |
16 ( 7 ) |
MAPK |
24 ( 8 ) |
| CDKN2A | 20 ( 5 ) | Insulin | 24 ( 5 ) |
*DEGs in response to CBHA were subject to IPA as detailed in “Material and Methods”. Gene pathway with total number of connections (direct and indirect) and focus genes are hierarchically arranged according to the number of focus genes. The relative position of the p53-specific network elicited at 6 h (11th) and 24 h (2nd) is significant.