Table 5.
Enriched transcription factor binding sites in the promoters of genes, obtained from H9c2 cells treated with CBHA or TSA*
|
CBHA 6h | ||||
|---|---|---|---|---|
| Transcription factors | # exp promoters hit | # exp promoters | P value | Q value |
| AP2_Q6 |
584 |
658 |
0 |
0 |
| CHCH_01 |
617 |
658 |
0 |
0 |
| E2F_Q2 |
548 |
658 |
0 |
0 |
| E2F1_Q3_01 |
317 |
658 |
0 |
0 |
| EGR2_01 |
592 |
658 |
0 |
0 |
| ETF_Q6 |
412 |
658 |
0 |
0 |
| GC_01 |
584 |
658 |
0 |
0 |
| KROX_Q6 |
411 |
658 |
0 |
0 |
| LRF_Q2 |
456 |
658 |
0 |
0 |
| MAZR_01 |
233 |
658 |
0 |
0 |
| NGFIC_01 |
434 |
658 |
0 |
0 |
| SP1_Q6_01 |
558 |
658 |
0 |
0 |
| WT1_Q6 |
528 |
658 |
0 |
0 |
| AHRHIF_Q6 |
361 |
658 |
1.00E-09 |
2.25E-08 |
| MUSCLE_INI_B |
593 |
658 |
1.93E-08 |
3.97E-07 |
| AHRARNT_02 |
336 |
658 |
1.54E-07 |
3.03E-06 |
| SPZ1_01 |
605 |
658 |
1.77E-07 |
3.35E-06 |
| AP2ALPHA_01 |
596 |
658 |
3.81E-07 |
6.21E-06 |
| AP2GAMMA_01 |
622 |
658 |
3.63E-07 |
6.21E-06 |
| MAZ_Q6 |
315 |
658 |
3.70E-07 |
6.21E-06 |
| UF1H3BETA_Q6 |
31 |
658 |
1.20E-05 |
1.89E-04 |
| CACBINDINGPROTEIN_Q6 |
431 |
658 |
1.26E-05 |
1.92E-04 |
| MOVOB_01 |
647 |
658 |
3.16E-05 |
4.44E-04 |
| VDR_Q3 |
603 |
658 |
3.42E-05 |
4.62E-04 |
| ZF5_B |
655 |
658 |
9.53E-05 |
1.25E-03 |
| E2F1DP2_01 |
293 |
658 |
1.48E-04 |
1.89E-03 |
| NFY_Q6_01 |
226 |
658 |
2.11E-04 |
2.62E-03 |
| CACD_01 |
635 |
658 |
2.61E-04 |
3.16E-03 |
| MZF1_02 |
571 |
658 |
3.58E-04 |
4.23E-03 |
| CACCCBINDINGFACTOR_Q6 |
78 |
658 |
4.56E-04 |
5.26E-03 |
| WHN_B |
145 |
658 |
4.92E-04 |
5.54E-03 |
|
CBHA 24h | ||||
| AP2_Q6 |
728 |
847 |
0 |
0 |
| AP2ALPHA_01 |
776 |
847 |
0 |
0 |
| CHCH_01 |
794 |
847 |
0 |
0 |
| E2F_Q2 |
680 |
847 |
0 |
0 |
| E2F1_Q3_01 |
379 |
847 |
0 |
0 |
| EGR_Q6 |
604 |
847 |
0 |
0 |
| ETF_Q6 |
492 |
847 |
0 |
0 |
| GC_01 |
737 |
847 |
0 |
0 |
| KROX_Q6 |
486 |
847 |
0 |
0 |
| MAZR_01 |
256 |
847 |
0 |
0 |
| NGFIC_01 |
532 |
847 |
0 |
0 |
| SP1_01 |
697 |
847 |
0 |
0 |
| WT1_Q6 |
682 |
847 |
0 |
0 |
| LRF_Q2 |
559 |
847 |
2.00E-10 |
4.68E-09 |
| MZF1_02 |
759 |
847 |
2.00E-10 |
4.68E-09 |
| SPZ1_01 |
780 |
847 |
1.80E-09 |
4.02E-08 |
| AP2GAMMA_01 |
802 |
847 |
3.60E-09 |
7.33E-08 |
| MOVOB_01 |
837 |
847 |
1.79E-08 |
3.49E-07 |
| CACBINDINGPROTEIN_Q6 |
555 |
847 |
8.55E-07 |
1.48E-05 |
| CACD_01 |
822 |
847 |
1.19E-06 |
1.99E-05 |
| CACCCBINDINGFACTOR_Q6 |
108 |
847 |
2.31E-06 |
3.72E-05 |
| AHRHIF_Q6 |
431 |
847 |
3.66E-06 |
5.53E-05 |
| NFY_Q6_01 |
294 |
847 |
1.24E-05 |
1.77E-04 |
| E2F1DP2_01 |
378 |
847 |
1.64E-05 |
2.26E-04 |
| AHRARNT_02 |
408 |
847 |
1.99E-05 |
2.67E-04 |
| MAZ_Q6 |
382 |
847 |
3.46E-05 |
4.51E-04 |
| STRA13_01 |
32 |
847 |
8.99E-05 |
1.08E-03 |
| SP3_Q3 |
821 |
847 |
3.89E-04 |
4.14E-03 |
| VDR_Q3 |
766 |
847 |
4.08E-04 |
4.24E-03 |
| MUSCLE_INI_B |
733 |
847 |
7.95E-04 |
8.10E-03 |
|
TSA 6h | ||||
| E2F_Q2 |
319 |
390 |
0 |
0 |
| E2F1_Q3_01 |
187 |
390 |
0 |
0 |
| ETF_Q6 |
243 |
390 |
0 |
0 |
| GC_01 |
341 |
390 |
0 |
0 |
| KROX_Q6 |
233 |
390 |
0 |
0 |
| SP1_Q6_01 |
320 |
390 |
0 |
0 |
| CHCH_01 |
361 |
390 |
8.00E-10 |
3.33E-08 |
| MAZR_01 |
128 |
390 |
1.70E-09 |
6.52E-08 |
| EGR2_01 |
350 |
390 |
1.66E-08 |
5.52E-07 |
| WT1_Q6 |
309 |
390 |
6.34E-08 |
1.98E-06 |
| NGFIC_01 |
249 |
390 |
8.85E-08 |
2.60E-06 |
| AP2_Q6 |
334 |
390 |
7.62E-07 |
2.00E-05 |
| LRF_Q2 |
262 |
390 |
8.74E-07 |
2.18E-05 |
| UF1H3BETA_Q6 |
20 |
390 |
8.70E-05 |
2.07E-03 |
| AHRHIF_Q6 |
205 |
390 |
9.40E-05 |
2.10E-03 |
| AHRARNT_02 |
195 |
390 |
1.99E-04 |
4.14E-03 |
| MAZ_Q6 |
183 |
390 |
2.74E-04 |
5.48E-03 |
| ZEC_01 |
2 |
390 |
3.48E-04 |
6.69E-03 |
|
TSA 24h | ||||
| GC_01 |
161 |
194 |
1.45E-05 |
2.49E-03 |
| NFY_01 |
118 |
194 |
9.66E-06 |
2.49E-03 |
| E2F_Q2 |
141 |
194 |
4.78E-05 |
4.94E-03 |
| SP1_Q2_01 | 143 | 194 | 8.48E-05 | 7.30E-03 |
* Overrepresented transcription factor binding sites were found in genes obtained from H9C2 cells induced with CBHA and TSA at different time points using CORE_TF. Differential expression of genes was considered to obtain the genes with 2 fold change.
* Experimental promoters hit shows the number of genes which have that TFBS in their promoter region, from the total number of genes scanned.
* One-tailed Mann–Whitney rank sum p value.
* Multiple testing corrected false discovery rate (FDR) q value, here q value <0.01.