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. 2013 Jan 31;8(1):e55612. doi: 10.1371/journal.pone.0055612

Figure 2. CSE induces the release of CXCL8, MMP8 and MMP9 from human PMNs.

Figure 2

(A) 105 freshly isolated buffy coat PMNs were stimulated for 9 hours with cigarette smoke extract (CSE; OD 0.03–0.24) or LPS (100 ng/ml; positive control). CXCL8, MMP8 and MMP9 ELISA's were performed on the supernatants. CSE induced the release of CXCL8 from fresh cells (** p<0.01 repeated measures ANOVA+Tukey CSE vs. control; ∧ p<0.01 paired t-test LPS vs. control). (B) CSE induced the release of MMP8 from fresh cells (* p<0.05 repeated measures ANOVA+Tukey CSE vs. control; ∧∧ p<0.01 t-test LPS vs. control). (C) CSE induced the release of MMP9 from fresh cells (* p<0.05 paired t-test CSE OD 0.12 vs. control; ∧∧ p<0.01 paired t-test LPS vs. control). Legend: each symbol represents a different donor (n = 5). Individual data are shown, horizontal bars represent mean values. The data presented here all passed the normality test.