Figure 4. Human PMN incubation with CSE does not affect the level and activity of PE in cell lysates.

(A) 10⁶ isolated PMNs were stimulated for 9 hours with CSE (OD 0.06 or 0.12). PE and GAPDH Western blots were performed on the cell lysates. PE in human neutrophils was a monomer and migrated at 75 kDa, which was similar to rhPE (not depicted). Incubation of PMNs with CSE did not change the optical density of the bands when compared to the control. (n = 2) (B) Freshly isolated PMNs (10⁶ cells) were stimulated for 16 hours with indicated reagents. PE activity was measured in lysates using Z-Gly-Pro-AMC as a substrate. Control was standardized to 1. Intracellular PE activity does not change after CSE exposure when compared to the control. (n = 5).