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AGS Cells were transfected with plasmid coding for VCP-Flag or empty vector, pretreated with MG-132 for 3 hr, and incubated for 6 hr with H. pylori. Immunoprecipitations were performed with anti-Flag M2 antibody affinity gel and the immunoprecipitates analyzed by immunoblotting to identify VCP interaction partners. 1% of input was subjected to immunoblotting.
