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The respiration protocol consisted of state IV respiration in the presence of 5 mM pyruvate+2 mM malate, the subsequent titration of ADP to maximal state III respiration (4 mM), and then the sequential addition of 10 mM glutamate and 10 mM succinate. Maximal uncoupled respiration was determined by titrating FCCP. Results represent means ± S.E.M.; n = 6 independent experiments.
