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. 2013 Jan 31;8(1):e53183. doi: 10.1371/journal.pone.0053183

Figure 14. ctnnb1Δex3 is produced in melanoblasts and SMC cells of E18.5 DA.

Figure 14

(A) The expression of β-catenin (bcat), Mitf-M (melanoblasts) and Hprt (loading control) was analyzed by RT-PCR on mRNA isolated from WT ( =  Tyr::Cre/°; +/+) and mut ( =  ctnnb1Δex3  =  Tyr::Cre/°; f3/+) DA at E18.5. M corresponds to the size marker. The “a” band (479 bp) corresponds to the non-recombined β-catenin cDNA or WT, whereas the “b” band (251 bp) corresponds to the recombined β-catenin cDNA or ctnnb1Δex3. Ptgs2 (Cox2) is weakly expressed in WT and mutant DA. (B) Schematic of the WT versus mut bcat amplicons. (C–H) Immunolocalization of β-catenin in red (C, E, F, H), β-galactosidase in green (D, E, G, H) and DAPI in blue (E, H) in WT-Dct (C–E) and ctnnb1Δex3-Dct (F–H) sections of E18.5 DA. Note that β-catenin is found in both the cytoplasm and the nucleus of ctnnb1Δex3-Dct DA.