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. 2013 Jan 31;8(1):e55226. doi: 10.1371/journal.pone.0055226

Figure 3. In vitro differentiation of hiPSCs generated on PCM-DM at early and late passages.

Figure 3

A) Embryoid bodies (EBs) on day 8, derived from hiPSCs generated on PCM-DM at early (Clone 1, passage 11; Clone 2, passage 12) and late (Clone 1, passage 22; Clone 2, passage 21) culture times. Scale bar = 200 µm. B) Quantitative RT-PCR analysis. Left: Expression levels of undifferentiated genes in EBs relative to the hiPSCs before differentiation. Right: Expression levels of lineage-specific genes in EBs relative to differentiated 201B7. Data are presented as the mean ± SD. C) Immunocytochemical staining of differentiated cells by culturing EBs on gelatin-coated chamber slides with DMEM containing 10% FBS for 1 week. Clone 1, iPS-DMC72-PCMDM01; Clone 2, iPS-DMC72-PCMDM02. Scale bar = 50 µm.