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. 2013 Jan 31;8(1):e54664. doi: 10.1371/journal.pone.0054664

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© 2013 Valencia-Cruz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells were previously treated with Cas II-gly at their corresponding IC, C = control cells without treatment, G = Cas II-gly treated cells. Western blot was done in the cytosolic portion after 6 h treatment for HeLa (panel A) and 2 h of treatment for CHP-212 (panel B), in order to detect active caspase-3, caspase-8 cleaved, cyt C, Bcl-2 and Bax. Protein expression was normalized to the -tubulin loading control. Each bar represents the mean SD (P0.05) from three independent experiments and expressed as Relative units measured by percentage of optical densitometry (% OD).

Inline graphic — Cells were previously treated with Cas II-gly at their corresponding IC, C = control cells without treatment, G = Cas II-gly treated cells. Western blot was done in the cytosolic portion after 6 h treatment for HeLa (panel A) and 2 h of treatment for CHP-212 (panel B), in order to detect active caspase-3, caspase-8 cleaved, cyt C, Bcl-2 and Bax. Protein expression was normalized to the -tubulin loading control. Each bar represents the mean SD (P0.05) from three independent experiments and expressed as Relative units measured by percentage of optical densitometry (% OD).