Figure 11. MitoSox
superoxide determination by flow cytometry in HeLa and CHP-212.
Superoxide radical was measured by flow cytometry with MitoSox (FL2-H channel) dye; SSCH corresponds to side scatter channel. Panel A: untreated HeLa cells, Panel B: Cas/HeLa, Panel C: untreated CHP-212 cells, panel D: Cas/CHP-212. Positive controls, are 3 h UV-irradiated cells. Data is representative of three independent experiments for each experimental condition.