Figure 4. DOCA or NaHCO₃ alone do not alter AE1 expression in kidney cortex.

The mRNA and protein abundance of AE1 in kidney cortex from mice treated with NaHCO₃ or DOCA alone was measured by real-time RT-PCR (A, B) and immunoblotting (C) in the kidney cortex. (D) Bar graphs summarizing data from immunoblotting. All data were normalized against β-actin obtained on the same immunoblotting membrane after stripping. Data are given as normalized mean ± SE; n = 5–7 animals/group.