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. 2013 Jan 31;8(1):e55168. doi: 10.1371/journal.pone.0055168

Figure 1. Schematic description of small RNA specific UPL-based quantitative PCR assay and our oligo design system.

Figure 1

Two steps small RNA specific UPL-based quantitative PCR assay relies on reverse transcription using small RNA specific stem-loop RT primer and real-time quantitative PCR reaction using small RNA specific forward primer, UPL21 probe and universal reverse primer (A). Workflow of our oligo design system (B). Primers and probe for the designed hsa-mir-181a specific UPL-based quantitative PCR assay (C).