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. 2013 Jan 31;8(1):e55249. doi: 10.1371/journal.pone.0055249

Figure 3. GM-CSF up regulates surface leptin receptor expression in peripheral neutrophils isolated from children.

Figure 3

A. Dose response effect of GM-CSF (0.1, 1, 10, 25 ng/ml) on leptin receptor expression on neutrophils from children (n = 3, P<0.01). B. Neutrophils incubated with GM-CSF (10 ng/ml), leptin (10 µg/ml), combination, or alone for 2 h were analyzed by FACS with mouse anti-human leptin mAb and mouse IgG2a isotype-matched control Ab. Mean fluorescence intensity (MFI) of anti-leptin receptor is plotted as fold increase compared to isotype control. (n = 7, data shown as mean ± SD) **p<0.01, *p<0.05 compared to freshly isolated group, one-way ANOVA; †p<0.05 compared to medium control, unpaired t test.