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. 2013 Jan 31;8(1):e55249. doi: 10.1371/journal.pone.0055249

Figure 4. GM-CSF mobilizes the intracellular pool of leptin receptor to the cell surface.

Figure 4

A.(a and b). Representative FACS data showing absence of surface leptin receptor expression in freshly isolated neutrophil. b. Immunofluorescence of leptin receptor on neutrophil (inset shows isotype control), also stained with PI for nuclei (red color). Intracellular pool of leptin receptor (green color) is observed from freshly purified neutrophils in which no expression of leptin receptor was observed by FACS. Isotype control antibodies were used as negative controls. B–E. Normalized neutrophil leptin receptor expression in fresh cells at 2 h in medium, GM-CSF, Cycloheximide (10 ng/ml), and GM-CSF+Cycloheximide, quantitated in F. *p<0.05, ***p<0.001 compared to unstimulated control, unpaired t test.