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. 2013 Jan 31;8(1):e53853. doi: 10.1371/journal.pone.0053853

Figure 1. Kinetics data of ESE-16 and the effects of ESE-16 on extracellular acidity in MDA-MB-231 cells after 24 h exposure.

Figure 1

A) Variation with concentration of ESE-16 of the reaction velocity (R1/[E]) of wild-type CAII and a mimic of CAIX as determined by the catalysis of 18O exchange. Wild-type CAII Ki = 569±61 nM and CAIX mimic Ki = 453±43 nM, calculated using the Henderson method for tight-binding inhibitors. B) Changes in extracellular pH of confluent MDA-MB-231 cells treated with the CAIX inducer, DFO, and ESE-16 and a combination of DFO and ESE-16. ESE-16 inhibited DFO-induced reduction in extracellular pH.