Figure 3. Hoechst 33342 and acridine orange-stained MCF-7, MDA-MB-231 and MCF-12A cells at 400× magnification after 24 h exposure.

Cells completing cell division are observed in vehicle-treated cells (A–C, G–I and M–O) while an increase in the number of cells blocked in metaphase is observed in and ESE-16-treated (200 nM) cells (D–E, J–L and P–R). Acridine orange staining appears to be more concentrated in actively dividing cells in both treated and untreated cells.