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| Gene (Accession No.) | Primer Sequence (5′ to 3′) | Product size (bp) | Tm (°C) |
| S100A4 (NM_002961) | F: GAGCTGCCCAGCTTCTTG | 124 | 59 |
| R: TGCAGGACAGGAAGACACAG | |||
| TIMP1 (NM_003254) | F: CCCTAAACTCTGCCGTCTCC | 159 | 60 |
| R: AGTGAGTTGCGGGGTTATGG | |||
| MMP9 (NM_004994) | F: AACCAATCTCACCGACAGGC | 154 | 60 |
| R: CAGATACGCCCATCACCACC | |||
| GAPDH (NM_003380) | F: CTCATGACCACAGTCCATGC | 155 | 53 |
| R: TTCAGCTCTGGGATGACCTT |
