Skip to main content
. 2013 Jan 31;8(1):e54804. doi: 10.1371/journal.pone.0054804

Figure 6. ATP enhances IL-23 production through an adenylate cyclase-dependent but not an ionotropic pathway.

Figure 6

(A) Overnight cultures of monocytes were pre-treated with ATP, Forskolin (FSK), dibutyrl cyclic AMP (dbcAMP) or calcium ionophore A23187 (CI) prior to application of LTA. Culture supernatants were harvested 24 h later and analyzed by ELISA for IL-23. (B) Monocytes were treated for 30 min with the adenylate cyclase inhibitor dideoxyadenosine (DDA) prior to 4h ATP treatment. Cells were then activated with LTA and 24 h supernatants analyzed by ELISA for IL-23. (C) Monocytes were pre-treated with calcineurin-dependent phosphatase inhibitor, cyclosporine A (CsA) for 30 min prior to 4 h treatment with CI (upper panel) or ATP (lower panel). Cells were then activated with LTA. After 24 h culture supernatants were removed and analyzed for IL-23 by ELISA. Data from all panels derived from 3 separate experiments with different donors +/− SEM.