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. 2013 Feb;98(2):163–171. doi: 10.3324/haematol.2012.070789

Figure 1.

Figure 1.

Expression analysis of Dlk1 in the mid-gestation embryo (A) Domain structure of the full-length Dlk1 protein. C, cytoplasmic domain; EGF, EGF-like repeat; JM, juxtamembrane domain; SS, signal sequence; TM, transmembrane domain. (B) Expression of Dlk1 mRNA isoforms in the E11.5 AGM region. Expression in 3T3-L1 cells served as a positive control. The asterisk indicates an extra PCR product of unknown identity that is likely the product of polymerase slippage in the repeat region. (C) Schematic diagram of an E11.5 embryo showing the relative positions of the sections in E-G. (D-G) Dlk1 transcript expression analysis by in situ hybridization on sections of an E11.5 embryo (D, 5x/0.15 objective) and the caudal (E), middle (F) and rostral (G) part of the AGM region (10x/0.25 objective). DA, dorsal aorta; FL, fetal liver; G, gut; HG, hindgut; LB, lung bud; M, myotome; NT, neural tube; UGR, urogenital ridges. (H,I) Immunohistochemical co-staining for Dlk1 [red, Cy3 in H and fluorescein isothiocyanate (FITC) in I] and (H) CD34 (green, FITC) or (I) smooth muscle actin, alpha (green, Cy3) on sections of E11.5 wild-type aortas. d, dorsal; v, ventral; scale bar is 20 μm.