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. 2013 Jan 31;8(1):e54931. doi: 10.1371/journal.pone.0054931

Figure 3. p140Cap binds directly to Csk through Csk SH2 domain. A.

Figure 3

A schematic representation of full length Csk kinase protein domains. B. A schematic representation of different Csk recombinant mutant proteins. C. HEK-293 cells transfected with GFP or GFP-p140Cap full length (p140 WT) were treated with 100 micromolar pervanadate solution as in Figure 2B. Cell extracts were immunoprecipitated with a specific antibody to p140Cap or a pre immune serum as negative control (Ctrl). Immunocomplexes run on 6% SDS-PAGE and transferred to nitrocellulose, were analysed by Far western blotting, incubating with the different Csk recombinant proteins and probing with antibodies specific for GST, Csk and p140Cap. The results are representative of three independent experiments.