Table 1. Phosphopeptides of p140Cap protein.
M (Da) | p140Cap phosphopeptides sequence | |
Ser 45 | 1580.7613 | 43-RFpSNVGLVHTSER-55 |
Ser 987 | 1208.5540 | 985-RGpSDELTVPR-994 |
Ser 1003 | 1593.7919 | 997-TEKPSKpSPPPPPPR-1010 |
Tyr 396 | 1825.9329 | 392-GEGLpYADPYGLLHEGR-407 |
In vivo phosphorylated p140Cap was immunoprecipitated from MCF7 human breast cancer cells and four phosphopeptides were recovered by liquid chromatography, analysed by mass spectrometry (MS) and subjected to MS2 and MS3 fragmentation. We indicate the position of phosphorylation sites within the NCBI Reference sequence NM_025248.2, the masses of the peptides (expressed in Da) and their amino acidic sequence.