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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biomaterials. 2013 Jan 11;34(10):2455–2462. doi: 10.1016/j.biomaterials.2012.12.026

FIG 1. P22 Capsid Expression System.

FIG 1

Co-expression of P22 coat (gp5) and GFP-scaffolding fusion (GFP/141–303 gp8) in BL21(DE3) results in the assembly of 60 nm diameter VLPs. Assembly is mediated by the C-terminus of the scaffolding protein and results in the incorporation of GFP into the closed VLP shell at an approximate ratio of 420 coat subunits to 300 scaffolding subunits. As previously described, the GFP-scaffolding fusion remains incorporated in the closed shell and demonstrates a mechanism of packaging non-native proteins into the P22 VLPs [13].