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Two derived lines (4E1, 1H2) serially passaged in Euprymna tasmanica for 500 generations and exhibiting no shift in percent NaCl where microbial growth initially or finally occurs (minimum 0.01 OD600) along a salinity gradient relative to ancestral Vibrio fischeri ES114 and unevolved JRM200. However, both 4E1 and 1H2 still exhibited changes in growth abundance and biomass (i.e., amplitude) along the gradient.
