(A) Colonization experiments with superior numbers of nonnative Vibrio fischeri ES114 (2:1, 5:1, 10:1) over native V. fischeri ET401 in Euprymna tasmanica. (B) Headstart advantage (12 h) of nonnative V. fischeri ES114 over native V. fischeri ET401. Total inoculum of both strains in artificial seawater is 1 × 103 CFUs/mL. Squid hatchlings were sacrificed at 48 h for enumeration of V. fischeri. Letters indicate significant differences using fixed-treatment analysis of variance (P < 0.05, α = 0.05).