Table 2.
Secondary antibodies used to label Drosophila brains
| Secondary antibody | Source | Dilution |
|---|---|---|
| Donkey anti-sheep Alexa488 | Molecular probes; A-11015 | 1:1000 |
| Donkey anti-mouse Alexa488 | Molecular probes; A-21202 | 1:1000 |
| Goat anti-rabbit Alexa488 | Molecular probes; A-11034 | 1:1000 |
| Donkey anti-mouse Alexa555 | Molecular probes; A-31570 | 1:1000 |
| Donkey anti-rabbit Alexa555 | Molecular probes; A-31572 | 1:1000 |
| Goat anti-guinea pig Alexa555 | Molecular probes; A-21435 | 1:500 |
| Goat anti-rat Alexa555 | Molecular probes; A-21434 | 1:500 |
| Donkey anti-mouse Alexa647 | Molecular probes; A-31571 | 1:500 |
| Donkey anti-rabbit Alexa647 | Molecular probes; A-31573 | 1:500 |
| Donkey anti-rat Cy5 | Jackson ImmunoResearch Europe; 712-175-153 | 1:400 |
| Goat anti-guinea pig Alexa647 | Molecular probes; A-21450 | 1:500 |