The positive, but not the negative regulatory effects of PI3K on src gating are mediated through Akt. As shown above, inhibition of ERK by U0126 or PI3K by LY294002 each partially prevented v-src gating of Cx43. Inhibition of Akt by Akti had the same effect as PI3K inhibition,, indicating that PI3K induced closure of Cx43 is mediated through Akt. By contrast, the “rescue” of src gating by inhibition of both ERK and PI3K was not reproduced by a combination of ERK and Akt inhibition, indicating that the negative influence of PI3K on Cx43 gating by src if mediated through a pathway other than Akt.