Table 1.
Cell line | hESO-ST-2 | hESO-ST-3 | hESO-ST-4 | hESO-ST-5 | hESO-ST-6 | hESO-ST-7 |
---|---|---|---|---|---|---|
Nuclear donor | Donor 1 | Donor 1 | Donor 1 | Donor 4 | Donor 3 | Donor 6 |
Cytoplast donor | Donor 2 | Donor 2 | Donor 2 | Donor 3 | Donor 4 | Donor 7 |
Fertilization | 2PN/2PB | 2PN/2PB | 2PN/2PB | 2PN/2PB | 3PN/1PB | 2PN/2PB |
Karyotype (passage no.) | 46 XX, P4 | 46 XX, P7 | 46 XY, P7 | 46 XX, P4 | 69 XXX, P4 | 46 XY, P3 |
Nuclear DNA origin (by STR) | Donor 1 | Donor 1 | Donor 1 | Donor 4 | Donor 3 | Donor 6 |
mtDNA origin | Donor 2 | Donor 2 | Donor 2 | Donor 3 | Donor 4 | Donor 7 |
mtDNA carryover (RFLP) | Undetectable | Undetectable | Undetectable | Undetectable | Undetectable | NT |
mtDNA carryover (ARMS-qPCR) | 0.20% | 0.01% | 1.70% | NT | NT | NT |
NT, not tested.