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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Nature. 2012 Dec 23;493(7434):684–688. doi: 10.1038/nature11738

Figure 1. Adoptive transfer system for GFP labeling MZ B cells.

Figure 1

(a) Frequency of CD35hiCD23lo MZ B cells amongst B220+ cells in CD19−/− mice before (left) or 8 wks after (right) transfer of GFP+ B cells. (b) Phenotype of CD19+ GFP+ B cells from A. Numbers indicate % of cells in gate. (c) In vivo anti-CD19PE labeling of MZ-phenotype (CD23loCD35hi) B cells. (d) Spleen section from mouse reconstituted with 2:1 mixture of non-tg and GFP+ B cells, stained with anti-GFP (green) and DAPI (blue). Marginal sinus is indicated by the dashed white line. (e) Spleen sections from the indicated mice that had received PE-IC (red) 16hr earlier, stained for CD169 (green) and CD35 (blue).