Arsenic trioxide inhibits ΔNp63 but not TAp63 expression in time- and dose-dependent manners.
A–C, Western blot analyses were prepared with extracts from ME-180 (A), HaCaT (B), and MCF10A (C) cells left untreated or treated with 10 μm arsenic trioxide for 3–24 h and then probed with antibodies against p63 and actin, respectively. D–F, Western blot analyses were prepared with extracts from ME-180 (D), HaCaT (E), and MCF10A (F) cells left untreated or treated with 1.0–20 μm arsenic trioxide for 12 h and then probed with antibodies against p63 and actin, respectively. G, Western blot analyses were prepared with extracts from MIA PaCa-2 cells, which were left untreated or treated with 5 μm arsenic trioxide for 3 to 12 h and then probed with antibodies against p63 and actin, respectively. H, the experiments were performed as in G except that MIA PaCa-2 cells were left untreated or treated with 1–10 μm arsenic trioxide for 12 h.