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. 2012 Dec 27;288(5):2907–2913. doi: 10.1074/jbc.M112.428607

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Knockdown of Pirh2 inhibits whereas ectopic expression of Pirh2 promotes arsenic-induced degradation of ΔNp63 protein. A and B, Western blot analyses were prepared with extracts from ME-180 (A) and HaCaT (B) cells that were transfected with scrambled siRNA or siRNA against Pirh2 for 3 days and then treated with 5 μm arsenic trioxide for 9 h. The blots were then probed with antibodies against Pirh2, ΔNp63, and actin, respectively. C and D. Western blot analyses were prepared with extracts from ME-180 (C) and HaCaT (D) cells transfected with pcDNA3 or pcDNA3–2×FLAG-Pirh2 for 2 days and then treated with 5 μm arsenic trioxide for 9 h. The blots were then probed with antibodies against the FLAG tag, ΔNp63, and actin, respectively.