TABLE 3.
Effect of pertussis toxin pretreatment on the expression of AGS3-Rluc and Gαi1-YFP in cells transfected with carboxyl-terminal truncated Ric-8A
RFU and RLU generated for the data set presented in Fig. 7C were measured as described under “Experimental Procedures” and expressed as % of control values observed in the absence of pcDNA3::Ric-8A transfection. Fig. 7C (left panel), RFU values for control and PT treatment were 57,640 ± 6,007 and 52,652 ± 4,983, respectively. Fig. 7C (left panel), RLU values for control and PT treatment were 613,083 ± 17,652 and 550,925 ± 19,720, respectively. Fig. 7C (right panel), RFU values for control and PT treatment were 105,582 ± 9,430 and 104,497 ± 10,406, respectively. Fig. 7C (right panel), RLU values for control and PT treatment were 357,334 ± 943 and 331,808 ± 13,914, respectively. Results are expressed as the mean ± S.E. of three independent experiments with triplicate determinations.
| Ric-8A-WT | Ric-8A-M1-N453 | Ric-8A-M1-N492 | ||
|---|---|---|---|---|
| Fig. 7C (left panel) | RFU | 206 ± 11 | 77 ± 1 | 150 ± 3 |
| RFU (PT) | 213 ± 11 | 89 ± 1 | 159 ± 10 | |
| RLU | 158 ± 11 | 145 ± 11 | 151 ± 5 | |
| RLU (PT) | 134 ± 2 | 140 ± 10 | 114 ± 4 | |
| Fig. 7C (right panel) | RFU | 528 ± 39 | 114 ± 7 | 313 ± 16 |
| RFU (PT) | 544 ± 23 | 129 ± 3 | 315 ± 9 | |
| RLU | 226 ± 25 | 154 ± 15 | 146 ± 3 | |
| RLU (PT) | 177 ± 8 | 160 ± 7 | 139 ± 8 | |