FIGURE 6.

The effect of Y747F and Y759F mutation of the β3 cytoplasmic domain on IGF1 signaling. a, the expression of integrin β3. Cells were cultured in DMEM with 10% FCS. Cell lysates were analyzed by Western blotting. Human integrin β3 (WT, Y747F, or Y759F) was stably expressed in CHO cells. b, cell survival in anchorage-independent conditions. CHO and β3-CHO (WT, Y747F, or Y579F) cells were plated in polyHEMA-coated plates, serum-starved in DMEM, and cultured with WT IGF1 (10 or 100 ng/ml) for 48 h. Cell survival was measured by MTS assays. The data are shown as mean ± S.E. (n = 6). Statistical analysis was performed using ANOVA and Tukey's multiple comparison test. c, intracellular signals induced by WT IGF1 in anchorage-independent conditions. p, phospho. Cells were plated in polyHEMA-coated plates and serum-starved in DMEM for 3 h. Cells were stimulated with WT IGF1 (100 ng/ml) for 30 min. Cell lysates were analyzed by Western blotting.