FIGURE 6.

OPN promoted U-87 MG cell migration and conferred resistance to cell apoptosis. U-87 MG cells were treated with different concentrations of OPN-FL, OPN-R, and OPN-L (0 nm, white; 1 nm, light gray; 10 nm, dark gray; and 100 nm, black) for 2 days. A, cell proliferation was determined by CKK-8 assay. B, VEGF production was determined by assaying VEGF concentration in the conditioned media by ELISA, and the overall value normalized by the cell number was determined in A. C, dose-dependent enhancement of U-87 MG cell motility by OPN using the transwell chemotaxis assay. D, U-87 MG cells adhered to BSA (white), OPN-FL (light gray), OPN-R (black), and OPN-L (dark gray) are protected against apoptosis induced by 10 μm thapsigargin or 30 μm WP-1066. E, U-87 MG cells adhered to BSA (white), OPN-FL (light gray), OPN-R (black), and OPN-L (dark gray) are protected against apoptosis induced by 30 μm WP1066, even in the presence of 10 μm LY294002 or 1 μm IKK VII, inhibitors of PI3K and IKK, respectively. Their overall viability was calculated by comparison with data from untreated cells. Data are shown as mean ± S.D. and calculated from at least three independent experiments. *, p < 0.05; #, p < 0.01 compared with BSA by one-way ANOVA with post hoc Dunnett's.