FIGURE 7.

OPN promoted T98G cell migration and conferred resistance to cell apoptosis in T98G cells. T98G and DBTRG-05MG cells were treated with different concentrations of OPN-FL, OPN-R, and OPN-L (0 nm, white; 1 nm, light gray; 10 nm, dark gray; and 100 nm, black) for 2 days. A, T98G. B, DBTRG-05MG. Cell proliferation was determined by CKK-8 assay. C, T98G. D, DBTRG-05MG. VEGF production was determined by assaying VEGF concentration in the conditioned media by ELISA, and the overall value was normalized by the cell number determined in A or B. E, dose-dependent enhancement of T98G cell motility by OPN-FL using the transwell chemotaxis assay. F, T98G cells adhered to BSA (white), OPN-FL (light gray), OPN-R (black), and OPN-L (dark gray) are protected against apoptosis induced by 15 μm thapsigargin or 10 μm WP-1066. Their overall viability was calculated by comparison with data from untreated cells. Data are shown as mean ± S.D. *, p < 0.05, compared with BSA by one-way ANOVA with post hoc Dunnett's.