FIGURE 6.

AdipoR1 and AdipoR2 internalization dynamics in response to adiponectin. A and C, shown are representative confocal images of DsRed-AdipoR1 (A)- or DsRed-AdipoR2-expressing (C) HEK293AD cells transfected with the recycling endosome marker transferrin receptor tagged with HA (green) and treated with 100 nm FLAdipoQ (left panels) or 100 nm GAdipoQ (right panels) for 0, 5, 30, and 60 min. Scale bars, 5 μm. B and D, shown is quantification of the colocalization index between DsRed-AdipoR1 or DsRed-AdipoR2 and transferrin receptor immunofluorescent signals in FLAdipoQ (left graphs)- or GAdipoQ-treated cells (right graphs). Data are presented as the average ± S.E. from at least 12 cells per group collected from 3 independent experiments.