FIGURE 1.

PHF6 localizes in nucleolus. A, PHF6 localized in nucleolus. HeLa cells transfected with control shRNA or PHF6-specific shRNA were fixed, and immunostaining was carried out using the indicated antibodies. B, validation of the specificity of PHF6 antibody and shRNA-mediated PHF6 knockdown efficiency. Stable PHF6 knockdown cells or control HeLa cells were lysed and subjected to Western blotting (WB) using anti-PHF6 antibody. C, PHF6 localization to nucleolus requires its PHD1 domain. HeLa cells were transfected with plasmids encoding SFB-tagged wild type or deletion mutants of PHF6. Cells were fixed and immunostained with anti-FLAG and anti-fibrillarin antibodies. A schematic diagram of PHF6 shows the PHD1 domain, PHD2 domain, and nuclear localization signal (NLS).