FIGURE 8.

CART 55–102 increases cell proliferation in INS-1 (832/13) cells via PKA-, MAPK-, and PKB-dependent pathways. The addition of 100 nm CART 55–102 increased proliferation in INS-1 (832/13) cells cultured in 25 mm glucose for 48 h. The addition of pharmacological inhibitors of PKA (H89), MEK1 (PD0325901), and PKB (Akti1/2 and MK2206) abolished the proliferative effect of CART. The data are presented as mean ± S.D. of n = 3–5 independent experiments each performed in replicates of 6–8 wells per condition. *, p ≤ 0.05 when compared with no CART control for each inhibitor.