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. 2012 Dec 18;288(5):3251–3264. doi: 10.1074/jbc.M112.405167

TABLE 1.

Summary of K2P constructs used in this study

Channels were tagged with eGFP fused to the N terminus, with or without an HA epitope tag in the external loop of the second pore-forming domain.

Tag Mutation Experiments
K2P3.1 rat channel
    rK2P3.1 None Wild type Electrophysiology
    rK2P3.1N53Q None N53Q
    GFP-rK2P3.1 eGFP Wild type Immunofluorescence
    GFP-rK2P3.1N53Q eGFP N53Q
    GFP-rK2P3.1-HA eGFP and HA Wild type Immunofluorescence, flow cytometry, and immunoblotting
    GFP-rK2P3.1N53QHA eGFP and HA N53Q

K2P9.1 rat channel
    rK2P9.1 None Wild type Electrophysiology
    rK2P9.1N53Q None N53Q
    GFP-rK2P9.1 eGFP Wild type Immunofluorescence
    GFP-rK2P9.1N53Q eGFP N53Q
    GFP-rK2P9.1-HA eGFP and HA Wild type Immunofluorescence, flow cytometry, and immunoblotting
    GFP-rK2P9.1N53QHA eGFP and HA N53Q