TABLE 1.
Summary of K2P constructs used in this study
Channels were tagged with eGFP fused to the N terminus, with or without an HA epitope tag in the external loop of the second pore-forming domain.
| Tag | Mutation | Experiments | |
|---|---|---|---|
| K2P3.1 rat channel | |||
| rK2P3.1 | None | Wild type | Electrophysiology |
| rK2P3.1N53Q | None | N53Q | |
| GFP-rK2P3.1 | eGFP | Wild type | Immunofluorescence |
| GFP-rK2P3.1N53Q | eGFP | N53Q | |
| GFP-rK2P3.1-HA | eGFP and HA | Wild type | Immunofluorescence, flow cytometry, and immunoblotting |
| GFP-rK2P3.1N53QHA | eGFP and HA | N53Q | |
| K2P9.1 rat channel | |||
| rK2P9.1 | None | Wild type | Electrophysiology |
| rK2P9.1N53Q | None | N53Q | |
| GFP-rK2P9.1 | eGFP | Wild type | Immunofluorescence |
| GFP-rK2P9.1N53Q | eGFP | N53Q | |
| GFP-rK2P9.1-HA | eGFP and HA | Wild type | Immunofluorescence, flow cytometry, and immunoblotting |
| GFP-rK2P9.1N53QHA | eGFP and HA | N53Q | |