TABLE 2.

Amino acid sequences identified by N-terminal sequencing analysis of the 130 kDa band detected in DEAE-enriched condition medium shown in Fig. 4A

Amino acid sequences were aligned by cycle order and identified by a BLAST protein search. Amino acid sequences shown in boldface type aligned with the 130-kDa fragment calculated from the C terminus.

Perlecan Protein core domain	Amino acid sequence	Amino acid positions	P1–P1′	Mast cell proteases
				Cathepsins	MMPs	Others
	GALLLA	7–12	A–G	S	2, 3
II	EAEFA	201–205	T–E
	SEGGR	457–461	T–S	G, L	2, 9, 11
III	VLQSLE	685–690	Q–V		2, 9, 13
	SPGQPS	1048–1053	P–S		2, 3, 9
IV	SVPLAA	1499–1504	S–S	S	2, 3
	AELLV	1759–1763	E–A	B, D, E, L	2, 3
	APSKP	1766–1770	R–A	B, L	1, 2
	PPQAR	1997–2001	L–P	D, E	2
	SASLA	2542–2546	S–S	S	2, 3
	SVHGP	3107–3111	L–S	D, E, G	9	Chymase
	ALGDP	3512–3516	L–A	D, G, S
	VELAD	3545–3549	H–V		2, 3
	VLLLV	3568–3572	H–V		2, 3
V	SVPLS	4060–4064	P–S		2, 3, 9