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. 2012 Dec 7;288(5):3346–3358. doi: 10.1074/jbc.M112.418046

FIGURE 2.

FIGURE 2.

Effect of Trx system on rtTF redox states and activity. A, hTrx1/TrxR-mediated oxidation and reduction of rtTF by NADP(H) is shown. The ratio of hTrx1/rtTF was ∼0.6:1. The formation or consumption of NADPH was measured by following an increase or a decrease in absorbance at 340 nm. B, shown is reduction of rtTF by hTrx1-TrxR-NADPH in a TF concentration-dependent manner. C, shown is oxidation of prereduced rtTF by hTrx1-TrxR-NADP+. The rtTF was treated with 10 mmol/liter DTT followed by dialysis to remove DTT before assay of NADPH formation. D, shown is the effect of hTrx1-TrxR-NADP(H) on rtTF-precoagulant activity. rtTF was incubated with hTrx1-TrxR-NADP(H) at room temperature for 2 h before assay for TF activity. The ratio of hTrx1/rtTF was ∼7:1. All data points in A–D are expressed as the mean ± S.D. (n = 3). *, p < 0.05; N.S., not significant.