FIGURE 3.

Effects of endogenous hTrx1/TrxR on cell-surface TF-procoagulant activity. A, a Western blot tests the effects of hTrx1 knockdown and selenium supplementation on the expression of hTrx1, TrxR, and TF. GAPDH was used as an internal control. B, shown are specific activities of cellular TrxR and Trx. Cell extracts had a protein concentration of 3 mg/ml, 20 μl of which was used in measuring TrxR/Trx activity via super-insulin assay. C, shown is the effect of elevating TrxR activity by selenium supplementation on the basal activity of cell-surface TF as well as on the response of cell-surface TF activity to H₂O₂. The concentration of H₂O₂ was 1 mmol/liter, and the treatment time of H₂O₂ was 15 min. D, shown is the effect of hTrx1 knockdown on cell-surface TF-procoagulant activity. All data points in B–D are expressed as the means ± S.D., n = 5. **, p < 0.01.