FIGURE 1.

ΔEGFR contains NES and NLS signals that are functional. A, schematic of EGFR depicting the deleted region of ΔEGFR as well as the location of the NLS and NES signals. SS, signal peptide; ECD, extracellular domain; TM, transmembrane domain; KD, kinase domain; CR, C-terminal regulatory region. B, confocal analysis of GFP-ΔEGFR and its mutants (green) in U87 cells. C, stable expression of V5-ΔEGFR and the mutants in U87 cells. D, biochemical analysis of U87 stable cells for nuclear V5-ΔEGFR. Quantitation and statistical analysis of Western blotting are shown in supplemental Fig. S4A. E, confocal analyses of U87 stable cells using V5 antibody (green). Nuclei were counterstained using DAPI (blue) for all confocal analyses. F, coimmunoprecipitation (IP) and Western blot analysis (WB) showing decreased association of ΔEGFR and ΔEGFR-NES with CRM1. Quantitation and statistical analysis of Western blotting are shown in supplemental Fig. S4B.