FIGURE 4.

ΔEGFR binding to human glioma genome. A, the distance of each peak in both directions from the TSS. B, transformed ChIP-Seq data for ΔEGFR and ΔEGFR-NES in the UCSC genome browser. Shown are the loci PHF20, C12ORF49, MTHFR, and SEC61A2. C, ChIP quantitative PCR analyses of selected genes in U87 stable cell lines. D, Real-time quantitative PCR analyses of selected genes in U87 stable cell lines. For all quantitative PCR experiments, values in the stable cell lines are shown relative to those in vector control cells (normalized to 1), and error bars represent mean ± S.E. from three biological replicates. E, Western blot analyses of selected genes in U87 stable cell lines. Quantitation and statistical analysis of Western blotting are shown in supplemental Fig. S8F. *, p < 0.05; **, p < 0.01; ***, p < 0.001.