FIGURE 5.

Neither MalE nor maltose facilitates the transition to the outward facing state. The steady state fluorescence emission spectra of pyrene-labeled MalFGK₂ recorded in the absence (black traces) or presence of ATP, MalE, and maltose (red traces) using the following pyrene-labeled MalFGK₂ transporters: MalK₈₃/MalK₈₃ (A), MalF₃₉₆/MalG₁₈₅ (B), and MalF₄₄₆/MalG₂₃₄ (C). The fluorescence spectra were recorded from 365 to 520 nm with excitation wavelength at 345 nm. The fluorescence spectra recorded around 465 nm are magnified in the insets.