FIGURE 6.

Transition kinetics toward the outward facing conformation. The time course fluorescence emission spectra for the pair MalK₈₃/MalK₈₃ were monitored after the addition of ATP, MalE, and maltose as indicated. The spectra were recorded at 375 nm to monitor pyrene monomers (A) and 460 nm to monitor exited state dimers (B). The fluorescence is expressed in arbitrary units as in Fig. 4.