TABLE 1.

The affinity of MalE for the transporter in nanodiscs determined by fluorescence quenching assay as previously described (16)

Vi, vanadate; Nu, nucleotide; NA, not applicable.

Transporter	Affinity for MalEa
	ATP + Vi	ATP + BMOE	No Nu	No Nu + BMOE	No Nu + maltose
	μm
Wild type	0.079 ± 0.009	1.41 ± 0.07	7.14 ± 1.13	5.71 ± 1.06	45.24 ± 19.32
MalK83-MalK83	0.046 ± 0.007	0.057 ± 0.006	6.93 ± 1.21	5.12 ± 0.74	NA
MalF396-MalG185	0.064 ± 0.011	0.073 ± 0.010	6.43 ± 1.42	4.32 ± 0.94	NA
MalF446-MalG234	0.085 ± 0.008	1.18 ± 0.13b	7.17 ± 1.65	13.73 ± 3.54	>45c

^a The data are derived from three independent measurements, and the fit is using a one-site binding equation.

^b The measurement was performed in the presence of Vi.

^c The measurement was performed using cross-linked MalF₄₄₆-MalG₂₃₄.