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ATPase activity of the pyrene-labeled transporters reconstituted in proteoliposomes
| Transporter | ATPase activity |
Transport activity |
||
|---|---|---|---|---|
| −MalE and maltose | +MalE and maltose | −MalE | +MalE | |
| nmol/min/mg | nmol/min/mg | |||
| Wild type | 11 ± 4 | 892 ± 91 | 0–0.1 | 2.3 ± 0.4 |
| MalK83-MalK83 | 9 ± 2 | 734 ± 65 | 0–0.1 | 1.8 ± 0.3 |
| MalF396-MalG185 | 12 ± 3 | 792 ± 84 | 0–0.1 | 1.7 ± 0.4 |
| MalF446-MalG234 | 7 ± 2 | 704 ± 73 | 0–0.1 | 1.5 ± 0.2 |
