FIGURE 5.
Sustained PKA activation in CnAβ−/− mice. A and B, white (WAT; A) and brown adipose tissues (BAT; B) of CnAβ−/− and CnAβ+/+ mice upon injection of β3-adrenergic receptor agonist CL (0.1 mg/kg) were determined at the times indicated. The data presented are representative of three independent experiments with two mice/group. C–E, white adipose tissues isolated from CnAβ−/− and CnAβ+/+ mice (n = 3) were challenged with β-adrenergic receptor agonist CL (1 μm) (C) or Iso (1 μm) (D and E) for the times indicated. The tissue extracts prepared were used to determine activation of PKA by immunoblotting analysis using phospho-PKA motif antibodies (phospho-PKA substrates) (C and D). E, effect of isoproterenol stimulation on phosphorylation of transcription factor CREB and HSL is also shown. Expression of tubulin is shown as a control.