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. 2012 Dec 20;288(5):3477–3488. doi: 10.1074/jbc.M112.419150

FIGURE 7.

FIGURE 7.

Increased cAMP accumulation and reduced phosphodiesterase activation in CnAβ−/− MEFs. A, the levels of cAMP in CnAβ−/− and CnAβ+/+ MEFs (n = 4) upon stimulation (+) or not (−) with Iso (10 μm for 5 and 15 min) were determined. The effect of the phosphodiesterase (PDE) inhibitor IBMX (0.5 mm) was also determined. B, PDE activity in CnAβ−/− and CnAβ+/+ MEFs (n = 3) upon stimulation (+) or not (−) with Iso (10 μm for 5 min) were determined. The effect of general PDE inhibitor IBMX (0.5 mm) was also determined. *, p < 0.05; #, p < 0.005. C, transcript levels of several PDE members in CnAβ−/− and CnAβ+/+ fat depots were determined by semiquantitative RT-PCR.